• Users Online: 21
  • Print this page
  • Email this page
Year : 2018  |  Volume : 6  |  Issue : 4  |  Page : 81-84

Antibacterial efficacy of herbal alternatives and 2% chlorhexidine on biofilm: An in vitro study

1 Department of Conservative Dentistry and Endodontics, Chhattisgarh Dental College and Research Institute, Sundara, India
2 Department of Conservative Dentistry and Endodontics, Maitri College of Dentistry and Research Centre, Anjora, India
3 Department of Oral Medicine and Radiology, Chhattisgarh Dental College and Research Institute, Sundara, Chhattisgarh, India

Correspondence Address:
Dr. Pooja P Bopche
Department of Conservative Dentistry and Endodontics, Bopche Chawl, Balaghat Road, Gondia 441614, Maharashtra.
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/INJO.INJO_1_19

Rights and Permissions

Aim: To evaluate the efficacy of natural agents for disinfection of root canal system, such as the effect of Azadirachta indica (neem), thyme oil, and 2% chlorhexidine gluconate (CHX) on multispecies biofilm of most commonly found pathogens such as Streptococcus mutans and Enterococcus faecalis in the field of endodontics. Materials and Methods: To visualize the antibacterial effectualness of the methanolic extracts of the medicated plants and CHX, an in vitro dentin model was used. A polymicrobic biofilm was grown on human extracted teeth for 21 days. Microbial load in the form of colony-forming units per milliliter (CFU/mL) after the antimicrobial treatment was calculated. Data were statistically analyzed using analysis of variance and Bonferroni post hoc tests. Results: Plant extracts showed remarkable antibacterial efficacy along with 2% CHX compared with that of control. A significant statistical difference was found with 2% CHX. Conclusion: The methanolic extract of A. indica, thyme oil, and CHX has shown considerable antimicrobial activity against polymicrobic dentinal biofilm of S. mutans and E. faecalis.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded74    
    Comments [Add]    

Recommend this journal